Abstract:
Inourstudiesontheeffectofdifferentmethodsofcryopreservationontheintegrityofthecytoplasmic membrane
of oocytes at metaphase of meiosis II revealed that the application of the balanced MPII oocyte
cryopreservation using DMSO as cryoprotectant 1.5 has seen a large number of oocytes with cytoplasmic
membrane damage, which can lead to cell death. The results showed that the most effective way to maintain
the integrity of the membranes of eggs after freezing and thawing method was found in the application of the
method of vitrification, in which there is the least percentage of damage of the cytoplasmic membrane of
oocytes.